艾可直播

Our current research interests are focused on the structure and dynamic membrane interactions in abnormal nerve myelin, in which alterations have resulted from experimental, pathological, or genetic conditions. To link structure and composition at the molecular, macromolecular and tissue levels in the nervous system, our studies in these areas have utilized x-ray diffraction, microscopy at the light and ultrastructural levels, a variety of protein and lipid biochemical techniques, and molecular modeling.

What holds myelin together, and how are its constituents targeted in diseases? To characterize the molecular organization of lipids and proteins in nerve myelin membranes, and to define the molecular interactions of its membrane arrays, we have correlated biophysical and biochemical results from different types of specimens (e.g., whole unfixed or fixed tissue from a phylogenetic range of vertebrates, tissue homogenates, tissue fractions and model lipid/protein systems). Among the questions we addressed were: how does the organization of proteins and lipids in myelin account for the inter-membrane interactions of the membranes? What is the structural basis of demyelination? Our findings are relevant to understanding the process of membrane disruption and delamination which occurs in demyelinating diseases such as multiple sclerosis and certain peripheral neuropathies. Our findings have also helped to explain how specific mutations in myelin adhesion proteins or in lipid biosynthetic pathways affect the stability and functionality of the myelin.

• Visigalli D, Capodivento G, Basit A, Fern谩ndez R, Hamid Z, Pencov谩 B, Gemelli C, Marubbi D, Pastorino C, Luoma AM, Riekel C, Kirschner DA, Schenone A, Fern谩ndez听 JA, Armirotti A, Nobbio L (2020) . Frontiers in Neurology: Neuromuscular Diseases. 11:903. doi: 10.3389/fneur.2020.00903
• Baumann A, Denninger AR, Domin M, Dem茅 B, Kirschner DA (2022) . BioRxiv doi: 10.1101/2021.12.17.473245v3. Current Research in Structural Biology 4:231-245. doi: 10.1016/ j.crstbi.2022.06.00
• Snow AD, Castillo GM, Nguyen BP, Choi PY, Cummings JA, Cam J, Hu Q, Lake T, Pan W, Kastin AJ, Kirschner DA, Wood SG, Rockenstein E, Masliah E, Lorimer S, Tanzi RE, Larsen L (2019) . Scientific Reports, Feb 6;9(1):561. doi: 10.1038/s41598-019-38645-0.
• Colombo L, Stoilova T, Deme B, Inouye H, Booth R, Del Favero E, Rondelli V,听Kirschner DA, Cantu L, and Salmona M (2017)听.听Scientific Reports, Jul 14;7(1):5510. doi:听10.1038/s41598-017-05582-9
• Inouye H, Kuo F-H, Denninger, AR, Weinhause B, Burghammer M,听Kirschner DA听(2017)听.听J Structural Biology, Dec;200(3):229-243. doi:听10.1016/j.jsb.2017.07.001
• Ahmed SS, Schattgen S, Frakes AE, Sikoglu EM, Su Q, Li J, Hampton TG, Denninger A,听Kirschner DA, Kaspar B, Matalon R, Gao G (2016)听.听Molecular Therapy听24(6):1030-41. doi: 10.1038/mt.2016.68. Epub 2016 Apr 4.
• Inouye H,听Kirschner DA听(2016)听.听Brain Research, Oct 28. pii: S0006-8993(15)00808-2. doi: 10.1016/j.brainres.2015.10.037.听
• Denninger AR, Breglio A, Maheras KJ, LeDuc G, Cristiglio V, Dem茅 B, Gow A,听Kirschner DA.听(2015)听.听Biophys J.听Oct 6;109(7):1387-1397. doi: 10.1016/j.bpj.2015.08.012. PubMed PMID: 26445439.
• Luoma AM, Kuo F, Cakici O, Crowther MN, Denninger AR, Avila RL, Brites P,听Kirschner DA听(2015)听.听Free Radicals in Biology & Medicine, 84:296-310. doi: 10.1016/j.freeradbiomed.2015.03.012.
• Heinecke KA, Luoma AM, d鈥橝zzo A,听Kirschner DA, Seyfried TN (2015)听.听ASN Neuro,听Jan-Feb 2015听7:听1759091415568913,听first published on听February 18, 2015;听doi:10.1177/1759091415568913.
• Denninger AR, Dem茅, B, Cristiglio V, LeDuc G, Feller WB,听Kirschner DA听(2014)听.听Acta Crystallographica D70:听3198-3211.听doi: 10.1107/S1399004714023815.
• Inouye H, Liu J, Makowski L, Palmisano M, Burghammer M, Riekel C,听Kirschner DA听(2014)听.听PLoS ONE听9(7):e100592. doi: 10.1371/journal.pone.0100592.听 http://hdl.handle.net/2345/bc-ir:103602
• Krishnan R, Tsubery H, Proschitsky MY, Asp E, Lulu M, Gilead S, Gartner M, Waltho JP, Davis PJ, Hounslow AM,听Kirschner DA, Inouye H, Myszka DG, Wright J, Solomon B, Fisher RA (2014)听.听Journal of Molecular Biology听426(13):2500-19. doi: 10.1016/j.jmb.2014.04.015. Epub 2014 Apr 22..
• Da Silva TF, Eira J, Lopes A, Malheiro AR, Sousa V, Luoma AM, Avila RL, Wanders R, Just W,听Kirschner DA, Sousa MM, Brites P (2014)听,听J Clinical Investigation听124(6):2560-70. doi: 10.1172/JCI72063. Epub 2014 Apr 24.
• Ahmed SS, Li H, Cao C, Sikoglu EM, Denninger AR, Su Q, Eaton S, Liso A, Xie J, Szucs S, Zhang H, Moore C,听Kirschner DA, Seyfried TN, Flotte TR, Matalon R, and Gao G听 (2013)听.听Molecular Therapy听21(12):2136-47. doi: 10.1038/mt.2013.138. Epub 2013 Jul 2.
• Patzk贸 A, Bai Y, Saporta MA, Katona I, Wu XY, Wrabetz L, Feltri ML, Wang S, Dillon LM, Kamholz J,听Kirschner DA, Sarkar F, Shy ME (2012)听.听Brain听135:3551-66.听 doi: 10.1093/brain/aws299.
• Saporta MAC, Shy BR, Patzko A, Bai Y, Pennuto M, Ferri C, Tinelli E, Saveri P,听Kirschner DA, Crowther M, Southwood C, Gow A, Wu X, Feltri ML, Wrabetz L, Shy ME (2012)听.听Brain听135:2032-2047.听 doi: 10.1093/brain/aws140.
• Jiang H, Kiebish MA,听Kirschner DA, Han X (2012) Multi-dimensional mass spectrometry-based shotgun lipidomics and its application for analysis of PEX7 mouse brain lipidome. In听Lipidomics 鈥� Technologies and Applications听(K Ekroos, ed.), Wiley-VCH, Weinheim, Germany.
• Gilardini A, Avila RA, Luckett R, Lu J, Oggioni N, Rodruguez-Menendez V. Bossi M, Canta A, Cavaletti G,听Kirschner DA听(2012)听.听NeuroToxicology 33:1-7.听 doi: 10.1016/j.neuro.2011.10.010.
• Echeverria V, Zeitlin R, Burgess S, Patel S, Barman A, Thakur G, Mamcarz M, Wang L, Sattelle DB,听Kirschner DA, Mori T, Leblanc R, Prabhakar R, and Arendash GW (2011)听.听J Alz Dis听23:1-19. (link to PubMed abstract).
• Avila RA, Inouye H, Feltri ML, Wrabetz L,听Kirschner DA听(2010)听. J Biol Chem, Oct 11. 285(53):42001-12.
• Inouye, H., Gleason, K.A., Decatur, S.M.,听Kirschner, D.A.听2010.听.听Proteins: Structure, Function, and Bioinformatics听78: 2306鈥�2321.
• Kirschner, D.A., Avila, R.L., Luoma, A., Enzmann, G.U., Agrawal, D., Inouye, H., Bunge, M.B., Kocsis, J.D., Peters, A., Whittemore, S. 2010.听.听Journal of Neuroscience Research听88: 712鈥�723.

• Verheijen, M.H., Camargo, N., Nadra, K., de Preux, A.-S., Verdier, V., Luoma, A.M., Crowther, M., Inouye, H., Shimano, H., Chen, S., Brouwers, J., Helms, B.J., Feltri, M.L., Wrabetz, L.,听Kirschner, D.A., Chrast, R., Smit, A.B. 2009.听.听Proceedings of the National Academy of Sciences of the United States of America听106: 21383鈥�8.
• Agrawal, D., Hawk, R., Avila, R.L., Inouye, H.,听Kirschner, D.A.听2009.听听Journal of Structural Biology听168: 521鈥�526.
• Kirschner, D.A., Karthigesan, J., Bizzozero, O.A., Kosaras, B., Inouye, H. 2009.听.听Neuron Glia Biology听4: 59鈥�70.
• Inouye, H., and听Kirschner, D.A.听2008. Myelin: A one-dimensional biological 鈥渃rystal鈥� for x-ray and neutron scattering. In:听Molecules: Aggregation, Nucleation, Crystallization听鈥�听Beyond Medical and Other Implications听(Eds. J. Sedzik, P. Riccio), World Scientific Publishing, Singapore.
• Flores, A.I., Narayanan, S.P., Morse, E., Shick, H.B., Avila, R.L.,听Kirschner, D.A., and Macklin, W.B. 2008.听.听The Journal of Neuroscience: The Official Journal of the Society for Neuroscience听28: 7174鈥�83.
• Kirschner, D.A., Gross, A.A., Hidalgo, M., Inouye, H., Gleason, K., Abdelsayed, G., Castillo, G.M., Snow, A.D., Pozo-Ramajo, A., Petty, S.A., and Decatur, S.M. 2008.听.听Current Alzheimer Research听5: 288鈥�307.
• Luo, X.Y., Cerullo, J., Dawli, T., Priest, C., Haddadin, Z., Kim, A., Inouye, H., Suffoletto, B.P., Avila, R.L., Lees, J.P., Sharma, D., Xie, B., Costello, C.E., and听Kirschner, D.A.听2008.听.听Journal of Structural Biology听162: 170鈥�183.
• Luo, X., Inouye, H., Sharma, D., Lee, D., Avila, R.L., Salmona, M., and听Kirschner, D.A.听2007.听.听Biophysical Journal听93: 3515鈥�3528.
• Luo, X.Y., Sharma, D., Inouye, H., Lee, D., Avila, R.L., Salmona, M., and听Kirschner, D.A.听2007.听.听Neurochemical Research听32: 197鈥�209.
• Yin, X., Baek, R.C.,听Kirschner, D.A., Peterson, A., Fujii, Y., Nave, K.A., Macklin, W.B., and Trapp, B.D. 2006.听.听Journal of Cell Biology听172: 469鈥�478.
• Wrabetz, L., D鈥橝ntonio, M., Pennuto, M., Dati, G., Tinelli, E., Fratta, P., Previtali, S., Imperiale, D., Zielasek, J., Toyka, K., Avila, R.A.,听Kirschner, D.A., Messing, A., Feltri, M.L., and Quattrini, A. 2006.听.听Journal of Neuroscience听26: 2358鈥�2368.
• Inouye, H., Sharma, D., Goux, W.J., and听Kirschner, D.A.听2006.听.听Biophysical Journal听90: 1774鈥�1789.
• Shinchuk, L. M., Sharma, D., Blondelle, S.Y., Reixach, N., Inouye, H., and听Kirschner, D.A.听2005.听听Proteins: Structure, Function, and Bioinformatics听61: 579鈥�589.
• Sharma, D., Shinchuk, L.M., Inouye, H., Wetzel, R., and听Kirschner, D.A.听2005.听.听Proteins: Structure, Function, and Bioinformatics听61: 398鈥�411.
• Avila, R.A., Inouye, H., Baek, R., Yin, X., Trapp, B.D., Feltri, M.L., Wrabetz, L., and听Kirschner, D.A.听2005.听.听Journal of Neuropathology & Experimental Neurology听64: 976鈥�990.
• Kirschner, D.A., Wrabetz, L., and Feltri, M.L. 2004. The P0 Gene. In:听Myelin Biology and Disorders 1, (Ed. R.A. Lazzarini et al.), Elsevier/Academic Press, NY, pp. 523鈥�545.
• Inouye, H., Bond, J.E., Deverin, S.P., Lim, A., Costello, C.E., and听Kirschner, D.A.听2002.听.听Biophysical Journal听83: 1716鈥�1727.
• Thompson, A.J., Cronin, M.S., and听Kirschner, D.A.听2002.听听Journal of Neuroscience Research听67(6): 766鈥�771.

2015听听听听听听听听

• 鈥淐IDP and Myelin Structural Biology鈥�, Invited lectures (12) as Visiting Professor in Department of Neurological, Neuropsychological, Morphological & Movement Sciences, University of Verona Medical School, Verona, Italy
• "From Macro to Nano: Focusing on Nerve Myelin鈥擭ature's Multilayer Membrane Assembly", Invited colloquium, Physics Department, 艾可直播 College (10/14)

2014听听听听

• 鈥淢yelin Structural Biology: Probing the Boundaries of Wrap鈥�, Invited talk, 艾可直播 Physics of Living Systems Hangout, MIT (4/10)

2013

• 鈥�@ & Between the Nodes: What X-rays & Neutrons Reveal About Nerve Myelin鈥�, Invited talk, IBMC, Porto, Portugal (5/9)

2012

• 鈥淣erve Myelin Between the Nodes: What X-rays & Neutrons Reveal鈥�, invited speaker, A.I. DuPont Hospital for Children, Nemours Translational Science Research Seminar Series, Wilmington, Delaware (10/25)
• 鈥淣eutron Diffraction from Myelin: Redux >Forty Years鈥�, invited speaker, Symposium: 鈥淪tructural Dynamics and Dynamical Structures: Future and Challenges of Neutron Scattering in Biology鈥�, Institute Laue-Langevin, Grenoble, France (10/3-10/5).
• 鈥淣ovel Molecular Views of Nerve Myelin Using Microbeam X-ray Diffraction and Neutron Diffraction鈥�, invited talk, American Crystallographic Association, annual meeting, 艾可直播 (7/30).
• 鈥淢yelin Structural Biology: Molecular Organization and Dynamic Interactions鈥�, Symposium on Membrane Biology, invited lecture, Acibadem University Medical School, Istanbul, Turkey (5/30)

2011

• 鈥淎 Sabbatical Report: 听Advances in Structural Biology: My Adventures in Milano & Grenoble听with X-rays & Neutrons, i Gelati & les Croissants, Ciao & Bonjour鈥�, seminar, Biology Dept, BC (11/1)
• 鈥淣erve Myelin:听 A New Rap On An Old Wrap 鈥� Pushing the Boundaries of Analysis鈥�, invited seminar, Department of Physiology and Biophysics, 艾可直播 University (10/18)
• 鈥淣ew Raps on A Phylogenetically Old Wrap:听 From Molecules to Myelinopathies鈥�, invited lecture at the BioCenter Oulu Doctoral Programme Advanced Course in Structural Biology, University of Oulu, Oulu, Finland (5/31)
• 鈥淓n(w)raptures of Myelin鈥�, invited lecture in 鈥淧rotein Structure & Disease鈥�, Brandeis University (3/28)

2010

• 鈥淎ssessing Myelin Development and Structure in Animal Models of Human Disorders 鈥� Istituto di Richerche Farmacologiche Mario Negri鈥�, Milano, Italy (10/11)
• 鈥淪tructure Determination by X-ray Diffraction鈥�, invited lecture in Biological Chemistry (Bi435), 艾可直播 College (4/30)